|Temple, Stephen - FORAGE GENETICS|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: July 9, 2001
Publication Date: September 15, 2004
Citation: Samac, D.A., Temple, S.J. 2004. Development and utilization of transformation in Medicago species. In: Liang, G.H., Skinner, D.Z., editors. Genetically Modified Crops: Their Development, Uses, and Risks. New York, NY: Haworth Press. p. 165-202. Technical Abstract: Transformation has been used as a tool for understanding physiology and development in Medicago species and for crop improvement. In this chapter, methods developed for efficient transformation and regeneration in M. sativa and M. truncatula are reviewed. The constitutive and tissue-specific promoters used in expression of transgenes in Medicago are described and the wide variety of traits that have been introduced for crop improvement summarized. Post transformation breeding strategies and issues regarding pollen flow in the development of a commercial genetically modified variety of alfalfa are illustrated in a summary of the development of Roundup Ready alfalfa.