United States Department of Agriculture Agricultural Research Service
 
Title: HIGH RESOLUTION TYPING OF TOXOPLASMA GONDII USING MICROSATELLITE LOCI

Authors
item Blackston, C - CDC, ATLANTA GA
item Dubey, Jitender
item Dotson, E - CDC, ATLANTA GA
item Su, C - WASH'TON UNIV,ST.LOUIS MO
item Tuilliez, P - INST PUERICULTURE, PARIS
item Sibleyd, - WASH'TON UNIV,ST.LOUIS MO
item Lehmann, T - CDC, ATLANTA GA

Submitted to: Journal of Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 25, 2001
Publication Date: N/A

Interpretive Summary: Infection by the protozoan (single-celled) parasite, Toxoplasma gondii, are widely prevalent in humans and anmals. It causes mental retardation and loss of vision in congenitally-infected children and abortion in livestock. There is a single species of the parasite in the genus Toxoplasma, T. gondii. Scientists at the Beltsville Agricultural Research Center and the CDC, Atlanta, Georgia describe a new high resolution system for typing strains of T. gondii. These results will be of interest to biologists, parasitologists and geneticists.

Technical Abstract: High resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current typing methods discern 3 lineages with minimal within-lineage variation. Described here are 6 new variable loci. These loci, including a minisatellite and 5 microsatellites, were more polymorphic than allozymes, RFLPs, and sequence variation in introns. Most importantly, these loci revealed, for the first time, substantial within-lineage variation that was over 6-fold higher than that detected by other markers. Genotyping at these loci facilitates classification of isolates beyond the lineage level.

     
Last Modified: 05/23/2013