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Title: CHARACTERIZATION OF RECOMBINANT SCFV ANTIBODY REACTIVE WITH AN APICAL ANTIGEN OF EIMERIA ACERVLINA (73RD NECAB MTG, COLLEGE PARK, MD)

Author
item MIN, WONGI - CHANGWON UNIV, KOREA
item KIM, JIN-KYOO - CHANGWON UNIV, KOREA
item Lillehoj, Hyun
item SOHN, EUN - USDA:ARS:BA:ANRI:PBESL
item HAN, JAE - SEOUL UNIV, KOREA
item SONG, KI - SEOUL UNIV, KOREA
item LILLEHOJ, ERIK - UNIV MD, COLLEGE PARK

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The chicken monoclonal antibody, 6D-12-G10, reacts with an apical complex protein at the anterior tip of E. acervulina sporozoites that inhibits parasite invasion in vitro. Because this mAb was produced at low amount from the original hybridoma cells, an scFv antibody was constructed by amplification of the corresponding VH and VL genes and expressed in E. coli. The scFv antibody was produced at a minimum of 7 mg/l and exhibited virtually identical antigen reactivity as the original mAb.