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United States Department of Agriculture

Agricultural Research Service

Title: Cloning and Characterization of Plant Parasitic Nematode Heterodera Glycines Genes by Using Caenorhabiditis Elegans Genome Information

Authors
item Kovaleva, Elena
item Masler, Edward
item Chitwood, David

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 6, 2001
Publication Date: N/A

Technical Abstract: The increased use of molecular biology techniques to study parasitic nematode physiology and biochemistry as well as parasite/host interactions opens up a number of possibilities to develop novel forms of effective and durable nematode controls. The genome of the soybean cyst-nematode Heterodera glycines, the major pathogen of soybean in the USA and world- wide, is similar in size and complexity (92.5 Mb, 82% unique) to the Caenorhabditis elegans genome, but less than 0.001% of it is so far sequenced. Using the large resource of C. elegans genetic information we have isolated several H. glycines genes with a conserved counterpart in C. elegans, known to play important roles in nematode development and adaptability. Here we describe three representatives: 1) actin, an intracellular component of cytoskeleton, ubiquitous and expressed at high level; 2) heat shock protein 70, molecular chaperone, expression of which depends on environmental stress reactions; 3) proprotein convertase 2, neuropeptide and hormone precursor processing endoprotease, known as a highly selective enzyme located in neuroendocrine cells with expression regulated during development. All three clones, isolated from a juvenile H. glycines cDNA library exhibit high homology to corresponding genes of C.elegans as well as other invertebrates. New H. glycines sequences were used to create phylogenic trees to study nematode diversity and evolutionary relationships among species on the molecular level. These new H. glycines sequences were used to further study nematode diversity, and evolutionary relationships among eukaryotes on the molecular level.

Last Modified: 8/27/2014
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