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United States Department of Agriculture

Agricultural Research Service

Title: Isolation and Characterization of White Lupin Proteoid Root Expressed Sequence Tags (Ests) Associated with Plant Hormones

Authors
item Uhde-Stone, C - UNIVERSITY OF MINNESOTA
item Li, A - UNIVERSITY OF MINNESOTA
item Daemen, M - INST HIGHER PROF EDUC
item Allan, D - UNIVERSITY OF MINNESOTA
item Vance, Carroll

Submitted to: International Congress on Plant Nutrition Proceedings
Publication Type: Proceedings
Publication Acceptance Date: July 27, 2001
Publication Date: N/A

Technical Abstract: White lupin (Lupinus albus L.), a nitrogen fixing organism that can acquire soil phosphorus (P) unavailable to other plants, develops proteoid roots (cluster roots) when grown in phosphorus deficient conditions. These are short, lateral, densely clustered rootlets that are adapted to increase P availability. To identify genes involved in the process of phosphorus adaptation, a cDNA library of white lupin proteoid roots was constructed. set of 1250 ESTs (expressed sequence tags) was identified by 5'sequencing. Based on homology to already known or predicted genes and proteins, a set of ESTs associated with the plant hormones auxin, cytokinin, and ethylene was identified. In comparison with +P normal roots, macroarray experiments and reverse RNA gel blots revealed differential expression of a number of hormone associated ESTs in -P proteoid roots.

Last Modified: 11/27/2014
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