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United States Department of Agriculture

Agricultural Research Service

Title: Resistance to Plum Pox Potyvirus in a Trangenic Woody Perennial Fruit Tree, European Plum (Prunus Domestica L.) Results from Post-Transcriptional Gene Silencing

Authors
item Scorza, Ralph
item Callahan, Ann
item Levy, L. - APHIS, ARS
item Damsteegt, Vernon
item Ravelonandro, M. - INRA-FRANCE

Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: May 15, 2001
Publication Date: May 31, 2001
Citation: Scorza, R., Callahan, A.M., Levy, L., Damsteegt, V.D., Ravelonandro, M. 2001. Resistance to plum pox potyvirus in a trangenic woody perennial fruit tree, European plum (Prunus domestica L.) results from post-transcriptional gene silencing. Acta Horticulturae 550:425-430.

Technical Abstract: Transgenic plums containing the plum pox potyvirus coat protein gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV and displayed characteristics typical of post-transcriptional gene silencing (PTGS) as described in herbaceous species, including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in th cytoplasm, and a complex multicopy transgene insertion.

Last Modified: 12/18/2014
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