Author
Scorza, Ralph | |
Callahan, Ann | |
LEVY, L. - APHIS, ARS | |
Damsteegt, Vernon | |
RAVELONANDRO, M. - INRA-FRANCE |
Submitted to: Acta Horticulturae
Publication Type: Proceedings Publication Acceptance Date: 5/15/2001 Publication Date: 5/31/2001 Citation: Scorza, R., Callahan, A.M., Levy, L., Damsteegt, V.D., Ravelonandro, M. 2001. Resistance to plum pox potyvirus in a trangenic woody perennial fruit tree, European plum (Prunus domestica L.) results from post-transcriptional gene silencing. Acta Horticulturae 550:425-430. Interpretive Summary: Technical Abstract: Transgenic plums containing the plum pox potyvirus coat protein gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV and displayed characteristics typical of post-transcriptional gene silencing (PTGS) as described in herbaceous species, including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in th cytoplasm, and a complex multicopy transgene insertion. |