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Title: PHYLOGENETIC ANALYSIS OF PRUNUS NECROTIC RINGSPOT VIRUS AND IMPLICATIONS FOR DEVELOPMENT OF A GENERALIZED TYPING ASSAY

Author
item HAN, L - AFFILIATION OF MPPL
item Hammond, Rosemarie

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 4/13/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Prunus necrotic ringspot virus (PNRSV), a member of the genus Ilarvius, occurs worldwide and is a serious pathogen of many woody plant species, including rose, many species of Prunus, and hops. Numerous strains exist which vary widely both biologically and serologically. A comprehensive collection of biological variants of PNRSV differing in geographic origin, host specificity, serology, and pathology were examined to establish the level of naturally occurring sequence variation. Alignment and phylogenetic analysis of the nucleotide sequence of the coat protein gene of 67 isolates confirmed the clustering of the isolates into three distinct groups that possess group-specific features. These features were used to develop diagnostic reagents for use in RT-PCR and PCR-ELISA for the detection and discrimination among virus serotypes and pathotypes in mixed infections. These standardized assays will be useful in monitoring the spatial and temporal spread of isolates in field studies.