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United States Department of Agriculture

Agricultural Research Service

Title: Molecular, Morphological and Thermal Characters of Pratylenchus Species (Nematoda: Tylenchida) and Relatives Using the D3 Segment of the Nuclear Ls28srrna Gene.

Authors
item CARTA, LYNN
item SKANTAR, ANDREA
item HANDOO, ZAFAR

Submitted to: Phytopathology Supplement; APSnet (Plant Pathology Online)
Publication Type: Abstract Only
Publication Acceptance Date: August 25, 2001
Publication Date: September 25, 2001
Citation: Carta, L.K., Skantar, A.M., Handoo, Z.A. 2001. Molecular, morphological and thermal characters of pratylenchus species (nematoda: tylenchida) and relatives using the d3 segment of the nuclear ls28srrna gene.. Phytopathology Supplement; APSnet (Plant Pathology Online) 91: 5131.

Technical Abstract: Gene sequences of the D3 segment of the LS28SrRNA gene generated by our lab and two others were aligned with the Clustal W program and evaluated by the PAUP program using parsimony and maximum likelihood methods. Congruence of trees with thermal, vulval and lip characters was evaluated. When Radopholus was the outgroup, and ambiguously-alignable positions excluded, tree topologies were congruent with morphological taxa possessing 2, 3 or 4 lip annules. An updated sequence for Pratylenchus hexincisus indicated it was an outgroup of P. penetrans, P. arlingtoni, P. fallax and P. convallariae. Pratylenchus zeae was a sister to P. hexincisus. The sisters of P. teres were P. minyus and Hirschmanniella belli rather than morphometrically similar P. crenatus. The P. agilis sequence is more closely related to the nearly identical sequences of P. pseudocoffeae and P. brachyurus than to P. scribneri, the originally diagnosed relative.

Last Modified: 9/10/2014
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