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United States Department of Agriculture

Agricultural Research Service

Title: Development of Simple Sequence Repeat (Ssr) Molecular Markers in Strawberry.

Authors
item Nourse Styan, Sarah
item Fickus, Edward
item Cregan, Perry
item Hokanson, Stan

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: April 25, 2001
Publication Date: N/A

Technical Abstract: Simple sequence repeat, SSR, molecular markers have been shown to be repeatable, co-dominant, highly polymorphic, technically simple to use, and well distributed within the genomes of many plant and animal species. SSRs developed for strawberry (Fragaria x ananassa Duchesne) should be of great utility for many applications including germplasm identification, genetic diversity assessment, QTL and gene mapping, and marker assisted selection. Genomic DNA from the strawberry cultivar Earliglow was digested with Sma1, Rsa1, and Msc1 restriction enzymes, electrophoresed, and DNA fragments of 400-800bp were selected. The DNA fragments were ligated into pUC19 plasmid cloning vectors and a genomic library was constructed. The library was screened with a P32 labeled (CT)15 probe. Forty (CT)n clones were sequenced, with twenty-seven of these clones containing suitable repeats and flanking regions for primer design. Twenty-two of theses primer sets have successfully amplified PCR products in a subset of cultivars and have been visualized on agarose and polyacrylamide gels. Work is ongoing to optimize the use of these SSR primers on various platforms including agarose gels, polyacrylamide gels, and fluorescent based automated detection systems.

Last Modified: 10/20/2014
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