ARS | Publication request: Development of Methods for Detection of Envelope Glycoprotein of Subgroup J Avian Leukosis Virus

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 14, 2001
Publication Date: July 14, 2001
Citation: LI, M., LEE, L.F., FADLY, A.M. DEVELOPMENT OF METHODS FOR DETECTION OF ENVELOPE GLYCOPROTEIN OF SUBGROUP J AVIAN LEUKOSIS VIRUS. AMERICAN VETERINARY MEDICAL ASSOCIATION ABSTRACT. 2001.

Technical Abstract: Subgroup J Avian leukosis virus (ALV-J) induces high incidence of myelo- cytomatosis in meat-type chickens. Successful control of ALV-J infection in breeder flocks is based on the sensitivity and specificity of methods used for detection of either antigens or antibodies to the virus. The gp85 envelope glycoprotein of strain ADOL-Hc1 of ALV-J was cloned into baculovirus and pET expression system for abundant expression of the protein. The expressed protein was purified and used to immunize rabbits to generate high titer antiserum to ALV-J. Using either monoclonal anti- bodies or rabbit anti-gp85 antibody, we detected gp85 antigens in infected chickens by indirect immunofluorescence test. Additional methods for detection of ALV-J antigens such as enzyme-linked immunoabsorbent assay or dot blot are being developed.