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United States Department of Agriculture

Agricultural Research Service

Title: Identification of Immunodominant Proteins of Neospora Caninum Nc-1 in 2 Dimensional Western Blots Using Serum from a Neospora Endemic Dairy Herd

Authors
item Hill, Dolores
item Scott, Alan - JOHNS HOPKINS, BALTO, MD
item Dyer, Robert - UNIV DELAWARE, NEWARK
item Dubey, Jitender

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2001
Publication Date: N/A

Technical Abstract: Vertical transmission of Neospora caninum has been documented in cattle, though the fidelity of transmission from dam to offspring is variable. The development of resistance to Neospora infection and transmission of N. caninum from dams to offspring may be affected by immunological recognition of specific N. caninum proteins by infected cattle. In this study, Neospora caninum tachyzoites were isolated from in vitro cell culture and proteins were extracted using a chaotropic detergent solution. Two- dimensional electrophoresis was carried out on the solubilized proteins using immobilized pH gradient (3-10) strips in the first dimension and 4-12% gradient slab gels in the second dimension. XProteins identified in Western blots by antisera from infected cows were cut from identical blots run in parallel and were subjected to N-terminal amino acid sequence analysis for identification. Twenty immunodominant proteins were identified using sera from N. caninum infected cattle. Four of the proteins were identified as surface associated proteins, 3 were identified as dense granule-associated proteins, 4 were identified as actin, myosin, or tubulin chains, 2 were identified as microneme associated proteins, and 3 were identified as cytoplasmic proteins.

Last Modified: 4/19/2014