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United States Department of Agriculture

Agricultural Research Service

Title: Analysis of a Peach Acc Oxidase Promoter by Transient Expression of the Promoter - Gus Fusions Via Particle Bombardment of Fruit Tissues

item Moon, Hangsik
item Srinivasan, Chinnathambi
item Scorza, Ralph
item Callahan, Ann

Submitted to: American Society of Plant Physiologists Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2001
Publication Date: July 21, 2001
Citation: Moon, H., Srinivasan, C., Scorza, R., Callahan, A.M. 2001. Analysis of a peach acc oxidase promoter by transient expression of the promoter - gus fusions via particle bombardment of fruit tissues. American Society of Plant Physiologists Meeting.

Technical Abstract: The phytohormone ethylene affects many aspects of plant growth and develop- ment. It is an important regulator of fruit ripening, leaf senescence and abscission, and of plant responses to environmental stimuli such as mechanical wounding, and pathogen infection. Ethylene is produced from S- adenosyl methionine by the action of the enzymes 1-aminocyclopropane-1- caboxylic acid (ACC) synthase and ACC oxidase. A genomic DNA encoding ACC oxidase was isolated from peach (Prunus persica L. Batsch). It has three introns and 2.95 kb of 5' flanking region. Previous work with its cDNA had shown that the accumulation of the mRNA occurred during the softening stage of fruit ripening and that this phenomenon was associated with increases in the amount of ethylene synthesized by fruits (Callahan et al., Plant Physiol., 100: 482-488). Efforts to genetically engineer fruit crops will benefit from the availability of fruit-specific promoters that specifically yaffect the fruit ripening process and no other tissues' development howeve relatively few fruit-specific promoters are currently available. A series of deleted promoters were fused to the uid gene (GUS) to identify specific functional regions in the peach ACC oxidase promoter that confer transgene expression in a tissue-specific manner at specific times during fruit development and ripening. The analyses of the expression of uid in transiently transformed fruit tissues via biolistic particle bombardment will be discussed.

Last Modified: 4/22/2015