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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Characterization of Red Rice (Oryza Sativa) and Rice (O. Sativa) Using Rapd Primers

Authors
item Estorninos, JR., L - UNIV ARK
item Burgos, N - UNIV ARK
item Gealy, David
item Talbert, R - UNIV ARK
item Stewart, J - UNIV ARK
item Sneller, C - UNIV ARK

Submitted to: Proceedings of Southern Weed Science Society
Publication Type: Abstract Only
Publication Acceptance Date: February 1, 2001
Publication Date: N/A

Technical Abstract: A study was initiated to analyze the genetic relationships within and between 23 red rice populations and three cultivated rice by DNA fingerprinting. Polymerase chain reaction (PCR) with fifteen 10-mer RAPD primers and genomic DNA template was used to generate amplified DNA fragments. The 15 RAPD markers produced a total of 111 reproducible bands, ,89 of which were polymorphic among red rice and cultivated rice populations, 78 were polymorphic among red rice, and 45 between cultivated rice. Clustering based on multidimensional scaling (MDS)showed general distribution of the 26 populations. Bengal, a medium grain rice cultivar, was categorized as Group I. The awned blackhull red rice, AR1994-8 and MS 1996-7, and awnless strawhull red rice, ARKaty-RR and AR 1996-1 were grouped as II, V, III, and IV, respectively, because they were genetically distant to each other and to the other clusters. The sixth group were the strawhull, awnless-type populations consisting of AR1994-7, MS1996-5, LA1995-12, AR-15A, AR1995-7, and ARSgt-S. Group VII consisted of awned blackhull red rice AR-17C and AR1995-1. Group VIII were awned strawhull types MS-4, LA-3, AR-11D, and LA1995-14, and awned blackhull types AR1995- 4, AR10A, AR-19A, AR-5A, and LA1995-13. Group IX consisted of small long grain cultivated rice Katy and Kaybonnet. Group X contained the awned blackhull ecotypes TX-4 and ARSgt-B. These results were in agreement with the general morphological groupings of most of the red rice accessions indicating that RAPD assay could be useful in discriminating among closely related species. The RAPD assay did not show clear evidence of outcrossing between rice and cultivated rice.

Last Modified: 11/25/2014
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