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United States Department of Agriculture

Agricultural Research Service

Title: Cryopreservation of Caribbean Fruit Fly (Anastrepha Suspensa) Embryos

Authors
item Wang, Wen - NORTH DAKOTA STATE UNIV
item Leopold, Roger
item HANDLER, ALFRED
item Freeman, Thomas - NORTH DAKOTA STATE UNIV

Submitted to: Abstracts 2000 Annual Meeting of Entomological Society Of America
Publication Type: Abstract Only
Publication Acceptance Date: March 1, 2001
Publication Date: N/A

Technical Abstract: Cryopreserving embryos of insects used in pest control programs such as the sterile insect technique allows the ability to store the germplasm of founder and back-up strains used in mass rearing, and the ability to store the germplasm of large numbers of genetic strains used for research purposes, thereby eliminating the frequent need to begin new generations of short-lived insects. Studies have been carried out on cryopreservation of Caribbean fruit fly Anastrepha suspensa embryos. Embryos incubated at 27 deg. C for 42 hours were used in the experiments. At this stage of development the critical steps of dorsal closure and head involution are completed and midgut convolutions appear. It was found that the embryos were permeable to water and cryoprotectant after treated in Hexane. Incubation of embryos in Schneider's cell culture medium following dechorionation and permeabilization afforded the highest hatching. The hatching rate for permeabilization, loading with 10% ethylene glycol and dehydration in vitrification solution treatment were 92.4%, 87.3% and 77% respectively, pupation rate from the larvae were 67.9%, 33.4% and 29.5% respectively, and adult emergence rate from pupae were 93.3%, 91.1% and 85.9% respectively. Up to 46.5% of embryos vitrified in liquid nitrogen vapor and kept in liquid nitrogen for 15 minutes hatched into larvae. An average of 28.9% of these larvae developed into pupae, and fertile adults were obtained from these pupae. Reproduction of the cryopreserved Caribbean fruit flies was examined by individually out crossing the adults to untreated flies and was found to be at control levels.

Last Modified: 9/29/2014
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