ARS | Publication request: MULTIPLE CAMPYLOBACTER COLI GENOTYPES FROM PIGS IN A FARROW-TO-FINISH SWINEOPERATION

Submitted to: International Animal Agriculture and Food Science Conference
Publication Type: Abstract Only
Publication Acceptance Date: July 24, 2001
Publication Date: N/A

Technical Abstract: Genotypes of Campylobacter coli isolates obtained from the feces of three sows, and rectal swabs from seventeen piglets from a farrow-to-finish swine operation were examined by pulsed field gel electrophoresis (PFGE). Five C. coli colonies were picked from a single culture plate for each sample following differential broth enrichment and growth on Campy-Cefex agar. Isolate genotypes were examined by PFGE following genomic DNA restriction endonuclease digestion with SmaI and SacII. Twenty-three SmaI and thirteen SacII genotypes were detected among ninety-nine C. coli isolates. One SmaI genotype was detected among isolates from each of two sows and each of seven piglets, two different genotypes were among isolates from one sow and each of seven piglets, and three different genotypes were detected in each of three piglets. Digestion with SacII revealed one genotype among isolates from each of two sows and eight piglets, two genotypes among isolates from each of eight piglets, and three genotypes among isolates from each of two piglets. Although some piglets were related to sows in the study and some were littermates, there was no discernable pattern of shared genotypes among piglets and their respective sows or between related piglets. Data indicate that the pigs had been exposed to multiple C. coli genotypes, and that detection of co- colonizing genotypes is possible by PFGE.