ARS | Publication request: DIFFERENTIATION BETWEEN MICROBIAL POPULATIONS OF SWINE FECES AND STORED MANURE BY GROUP SPECIFIC AMPLIFIED RDNA RESTRICTION ANALYSIS

Submitted to: Microbial Ecology International Symposium
Publication Type: Abstract Only
Publication Acceptance Date: August 30, 2001
Publication Date: N/A

Technical Abstract: Anaerobic digestion of swine manure during storage results in the production of various odorous compounds. Although this is the result of microbiological activity, little is known about microorganisms involved in these processes. Group specific amplified ribosomal-DNA restriction analysis (GS-ARDRA) was evaluated as a method to determine changes in microbial community structure among swine fecal and stored manure samples. PCR primer sets were developed targeting Bacteroides-Prevotella, clostridia clusters IX & XI, clostridia clusters XIVa & XIVb, clostridia clusters I & II, Lactobacillus, Desulfovibrionaceae, and Streptococcus-Lactococcus groups as well as a universal primer pair. Seven tetrameric restriction enzymes were screened for their ability to differentiate among organisms within target groups, in order to optimize a set of at least three restriction enzymes for each target group. Only the restriction enzyme Msp I was suitable for all target groups and four restriction enzymes were used except for clostridial cluster I & II group where only three restriction enzymes were found to be suitable. Differentiation among swine fecal and stored manure samples using GS-ARDRA was possible with all target groups with some restriction enzymes.