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United States Department of Agriculture

Agricultural Research Service

Title: Linkage Mapping of Four Genes (Otc, Tbg, Slc25a5 and Fmr1) on Porcine Chromosome X

Authors
item Cepica, S - ACAD. SCI. CZECH REPUBLIC
item Rohrer, Gary
item Knoll, A - ACAD. SCI. CZECH REPUBLIC
item Masopust, M - ACAD. SCI. CZECH REPUBLIC
item Malek, O - ACAD. SCI. CZECH REPUBLIC

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 22, 2001
Publication Date: N/A

Interpretive Summary: Identification of genes accounting for significant effects on economically important traits ultimately requires alignment of the human and swine maps. Until now only a few genes have been mapped by linkage analysis on porcine chromosome X. Though the genes mapped to chromosome X are the same in such distinct species as human and mouse, the relative orders of genes are frequently quite different. Therefore to determine if the order of genes on the pig and human X chromosomes are the same we mapped four new genes to add to the five other genes previously mapped. Even after the addition of four genes to the porcine linkage map there is no evidence of rearrangements in gene order between porcine and human X chromosomes. Of newly mapped genes only TBG can be considered a possible candidate gene as it maps to the near proximity of QTL for fatness.

Technical Abstract: For amplification of the porcine gene fragments amplification primers were designed from human gene sequence for OTC, TBG, SLC25A5 and FMR1. The PCR products of expected length were observed on agarose gels and their identities were confirmed by partial terminal sequencing. Comparative positional candidate cloning of genes accounting for significant genetic variation of ETLs ultimately requires alignment of the human and swine maps for which linkage mapping of comparative (type 1) markers is essential. Until now only CALB3, HPRT1, F9, AR and PGK1 had been mapped by linkage analysis on porcine chromosome X. Though the genes mapped to chromosome X are the same in such distinct species as human and mouse, the relative orders of gene homologs are frequently changed by interstitial inversions. At least six homology segments rearranged by inversions have been found between human and mouse X chromosomes, while human and feline gene order are identical. Even after the addition of four genes to the porcine linkage map there is no evidence of rearrangements in gene order between porcine and human X chromosomes. Of the newly mapped genes only TBG can be considered a putative comparative positional candidate gene as it maps to the near proximity of QTL for fatness.

Last Modified: 10/24/2014