Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 20, 2001
Publication Date: N/A
Technical Abstract: Bovine viral diarrhea virus (BVDV) is a ubiquitous virus of cattle world-wide. Strains of BVDV can cause disease from clinically inapparent to severe-acute hemorrhagic syndrome that has a high mortality rate. Serial analysis of gene expression (SAGE), a sequence based technology that allows the global analysis of gene expression, was used to examine gene expression changes in BVDV-infected cells. SAGE libraries were constructed from mRNA purified from mock-infected and virulent BVDV2 strain 1373-infected MDBK cells. A total of 53,068 SAGE tags were sequenced, 26,611 and 26,457 from the mock-infected and BVDV2-1373-infected libraries, respectively. The SAGE tags were used to identify the bovine genes from which they were derived using BLAST searches of GenBank and the bovine EST databases. Approximately 200 bovine genes were identified and the expression profiles were compared between libraries. Expression of genes encoding structural and mitochondrial proteins were down-regulated (> 5-fold), indicating alterations in the cell cytoskeleton as well as a decrease in energy production. Expression of genes encoding proteins involved in protein synthesis were up-regulated (>5-fold) as were genes encoding proteins involved in nascent protein transport into the endoplasmic reticulum and in detoxification. A number of SAGE tags that showed significant changes in expression level between libraries have not yet been identified and are the focus of ongoing studies.