Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: May 16, 2001
Publication Date: N/A
Technical Abstract: Silver ion high performance liquid chromatography (Ag-HPLC) has proven itself to be a very versatile procedure for the separation and isolation of cis and trans geometric and positional fatty acid methyl ester (FAME) and triacylglycerol (TAG) isomers. Ag-HPLC has been applied to the separation/quantitation of cis and positional isomers from partially hydrogenated vegetable oils, conjugated FAMEs, FAMEs labelled with deuterium atoms on the double bond carbons, TAG isomers and to the separation of FAME or TAG mixtures containing fatty acids of widely differing chain lengths. We applied Ag-HPLC [Varian ChromSpher Lipids (c); dual-column; acetonitrile (ACN) in hexane as solvent; UV and RI detection] to the analysis of tailored lipids (TAGs with ABA and AAB configuration) synthesized by our group and found the method to be a suitable alternative to and more rapid than enzyme- or gas chromatography-based techniques. The analysis of di- and triacylglycerol mixtures by Ag-HPLC was not possible at room temperature (ca. 231 C) due to the limited solubility of the DGs in the ACN/hexane solvent system. The use of more polar solvents such as methanol or isopropanol resulted in gradual loss of column efficiency.