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United States Department of Agriculture

Agricultural Research Service

Title: Direct Interaction of Resistance Gene and Avirulence Gene Products Confers Rice Blast Resistance

Authors
item Jia, Yulin
item Bryan, G - DUPONT AG PROD
item Farrall, L - DUPONT AG PROD
item Wu, K - CEREGEN
item Hershey, P - DUPONT AG PROD
item Mcadams, S - DUPONNT AG PROD
item Tarchini, R - DUPONT AG PROD
item Faulk, K - DUPONT AG PROD
item Donaldson, G - DUPONT AG PROD
item Valent, B - DUPONT AG PROD

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: August 14, 2000
Publication Date: N/A

Technical Abstract: Rice blast is a classical gene-for-gene system. Resistance occurs when the rice Pi-ta protein responds to expression of a fungal M. grisea avirulence gene AVR-Pita. We have isolated this matched pair of resistance and avirulence genes by map-based cloning. AVR-Pita is predicted to encode a processed metalloprotease. Pi-ta encodes a putative cytoplasmic protein with a nucleotide binding site and a leucine rich domain. A single nucleotide substitution of A918 with S in a natural allele of Pi-ta has resulted in loss of resistance. We demonstrated that Pi-ta bound AVR-Pita in the yeast two-hybrid system and in vitro binding. We observed that transient expression of AVR-Pita inside rice cells elicited a Pi-ta dependent hypersensitive cell death. Taken together, our data suggest that Pi-ta is an intracellular receptor that binds specifically to a fungal molecule and then activates its defense system, and that A918 is critical for recognition specificity.

Last Modified: 9/22/2014
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