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United States Department of Agriculture

Agricultural Research Service

Title: A Selected Sct of Trinucleotide Simple Sequence Repeat Markers for Soybean Cultivar Identification

Authors
item Cregan, Perry
item Quigley, Charles
item Nelson, R - URBANA, IL
item Carter, T - RALEIGH, NC
item Song, Q - VISITING SCIENTISTS
item Boerma, H - UNIV. OF GEORGIA
item Strachan, J - USDA, AMS, BELTSVILLE, MD

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: November 5, 2000
Publication Date: N/A

Technical Abstract: Soybean cultivars are described for purposes of Plant Variety Protection (PVP) by pigmentation and morphological traits. However, many commercial soybeans arise from a limited number of elite lines are often indistinguishable based on the traits. The purpose of the work was to select a small set of trinucleotide SSR that would provide a high level of discriminatory power to distinguish soybean genotypes. A total of 48 fluorescently labelled SSR primer sets was used to amplify DNA of the 35 ancestors of N. American and a diverse group of elite N. American cultivars. Analysis resulted in the identification of a subset of 13 loci, from 12 different linkage groups, that easily produced unique SSR allele size profiles for each of the 66 elite N. American cultivars. This set of 13 loci was used to characterize four independent sets of elite cultivars that were selected based upon identical maturity and morphological traits. Based upon these analyses, all cultivars could be distinguished using the set of 13 selected loci. This set of loci is proposed as a standard set for use in DNA profiling for purposes of obtaining PVP.

Last Modified: 12/21/2014
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