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United States Department of Agriculture

Agricultural Research Service

Title: Summary of Workshop Findings for Porcine Myelomonocytic Markers

Authors
item Thacker, E - IOWA STATE U AMES
item Ezquerra, A - BRISTOL UK
item Dominguez, J - MADRID, SPAIN
item Alonso, F - MADRID SPAIN
item Haverson, K - BRISTOL UK
item Lunney, Joan
item Mccullough, K - SWITZERLAND
item Summerfield, A - SWITZERLAND

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 25, 2001
Publication Date: N/A

Interpretive Summary: Effective responses to disease and vaccines require the interaction of many cells in the immune system. Cells of the myelomonocytic lineage, the macrophages and monocytes, play an especially important role in the immune response and induction of inflammation in swine because they help to identify the foreign antigen and initiate cellular interactions which control the exact nature of the immune or inflammatory response that occurs. This manuscript presents data from the Third International Swine Cluster of Differentiation (CD) Workshop. For this Third Swine CD Workshop the reactivity of a panel of 65 test monoclonal antibodies (mAb) against cells of myeloid origin was analyzed. After comparing the assay data, clustering analysis and inhihition studies, one mAb that recognized macrophages and neutrophils was assigned to swine workshop cluster number 3 (SWC3). A second mAb was assigned to SWC8, and mAb reactive the CD14 antigen on porcine myelomonocytic cells and three different CD11 antigens were also identified. These mAb will enable swine researchers to better define the cellular interactions that control disease and inflammatory responses.

Technical Abstract: Sixty-five monoclonal antibodies (mAb) including seventeen internal controls were analyzed for their ability to recognize and bind to various cells of the myelomonocytic lineage. Flow cytometry (FCM), utilizing both single and double staining, and immunoprecipitation assays were used in the analysis. Thirty-eight of the mAb were determined to be reactive with myelomonocytic cells resulting in nine clusters of interest. Additional mAb were assigned to the SWC3, SWC8 and CD14 clusters. Although the exact identity of many of the molecules on the cells bound by the mAb remains undetermined, the information obtained about the mAb analyzed in this workshop should be helpful in further identifying various populations of myelomonocytic cells and their stages of differentiation. Despite the fact that further work is required to determine the antigens recognized by many of the mAb, it was determined that 12 mAb possessed possible CD11 specificity, while other mAb could be assigned to SWC3, SWC8 or CD14 specificities.

Last Modified: 10/20/2014
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