|Lee, Margie - UNIVERSITY OF GEORGIA|
|Maurer, John - UNIVERSITY OF GEORGIA|
|Buckner, Leanne - UNIVERSITY OF GEORGIA|
Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2001
Publication Date: July 1, 2001
Citation: LEE, M., MAURER, J., BERRANG, M.E., BUCKNER, L. CLASS 1 INTEGRON-ASSOCIATED ANTIBIOTIC RESISTANCE CASSETTES IN CAMPYLOBACTER JEJUNI ISOLATED FROM BROILER CHICKENS. AMERICAN SOCIETY FOR MICROBIOLOGY. 2001. Technical Abstract: Campylobacter jejuni is one of the leading causes of foodborne enteritis in the US. While most infections are self-limiting, invasive infections and chronic colitis are commonly treated with antibiotics. Antibiotic resistance is increasingly a serious problem in some pathogens and the capability for multidrug resistance is a very real concern. Integrons are genetic elements that can mediate multidrug resistance in many gram- negative bacteria,including Campylobacter. Using a combination of PCR and DNA/DNA hybridization, we have screened over 150 C. jejuni, isolated from chicken feces or broiler carcass washes, for the presence of class I integrases. Positive isolates were probed for the presence of known class I integron gene cassettes which confer aminoglycoside, beta- lactam, chloramphenicol,and tribrissen resistance. Only about 20% of isolates contained class I integrase and most did not contain known gene cassettes. Cassettes conferring aminoglycoside resistance were most commonly detected.