Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2001
Publication Date: July 1, 2001
Citation: LEE, M., MAURER, J., BERRANG, M.E., BUCKNER, L. CLASS 1 INTEGRON-ASSOCIATED ANTIBIOTIC RESISTANCE CASSETTES IN CAMPYLOBACTER JEJUNI ISOLATED FROM BROILER CHICKENS. AMERICAN SOCIETY FOR MICROBIOLOGY. 2001.
Campylobacter jejuni is one of the leading causes of foodborne enteritis in the US. While most infections are self-limiting, invasive infections and chronic colitis are commonly treated with antibiotics. Antibiotic resistance is increasingly a serious problem in some pathogens and the capability for multidrug resistance is a very real concern. Integrons are genetic elements that can mediate multidrug resistance in many gram- negative bacteria,including Campylobacter. Using a combination of PCR and DNA/DNA hybridization, we have screened over 150 C. jejuni, isolated from chicken feces or broiler carcass washes, for the presence of class I integrases. Positive isolates were probed for the presence of known class I integron gene cassettes which confer aminoglycoside, beta- lactam, chloramphenicol,and tribrissen resistance. Only about 20% of isolates contained class I integrase and most did not contain known gene cassettes. Cassettes conferring aminoglycoside resistance were most commonly detected.