ARS | Publication request: AN INITIAL GENETIC LINKAGE MAP FOR WATERMELON BASED ON RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 25, 2000
Publication Date: January 12, 2001
Citation: Levi, A., Zhang,, X., Thomas, C.E., Joobeur, T. 2001. An initial genetic linkage map for watermelon based on randomly amplified polymorphic dna (rapd) markers. Plant and Animal Genome Conference Proceedings. (S03-01) (#666).

Technical Abstract: An initial genetic linkage (RAPD-based) map has been constructed for watermelon using a BC1 population [U.S. Plant Introduction accession 296341-FR (Fusarium resistant) x New Hampshire Midget] x New Hampshire Midget. The map contains 142 RAPD markers that segregate in 15 linkage groups and cover a genetic linkage distance of 1165.8 cM. Among these RAPD markers is Operon-P-01 (700 bp) that was reported as linked (3 cM) to race 2 Fusarium wilt resistance in watermelon. Each of the large linkage groups contains segments with low levels of recombination among markers (0-2.6 cM) indicating that the watermelon genome may contain large conserved chromosomal regions. BC1-F2 families are being generated and will be used to identify markers closely linked to genes that control fruit quality and race 2 Fusarium wilt resistance in watermelon.