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United States Department of Agriculture

Agricultural Research Service

Title: A Monoclonal Antibody Based Immunoaffinity Column for Isolation of Pcdd/ Pcdf from Serum.

Authors
item Shelver, Weilin
item Huwe, Janice
item Stanker, Larry
item Patterson, Donald - CDC
item Turner, Wayman - CDC

Submitted to: Organohalogen Compounds
Publication Type: Proceedings
Publication Acceptance Date: July 20, 2000
Publication Date: N/A

Interpretive Summary: Environmental contaminants such as polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and polychlorinated biphenyls (PCBs) are present in trace amounts, in complicated matrices, and can bioaccumulate through the food chain. Analysis of these compounds requires lengthy cleanup procedures prior to analysis by sophisticated instrumental methods. Consequently, the costs of analyzing such chemicals are prohibitively high, about US $1,000 per sample. To ensure food safety, a faster and economical analysis for PCDDs/PCDFs/PCBs is needed in order to analyze large number of samples. Our laboratory has developed a method that uses an antibody's specific binding sites as a tool to separate the PCDDs and PCDFs from interferences. This method can decrease toxic organic solvent usage and the steps needed for sample cleanup, thus shortening cleanup times and decreasing the cost of analysis.

Technical Abstract: Polychlorinated aromatic compounds such as dioxins, dibenzofurans, and PCBs are ubiquitous environmental contaminants. To detect the trace of dioxins/dibenzofurans instruments such as high resolution GC-high resolution MS are needed. Consequently, current dioxins analysis is very costly, at a cost of US$1000/sample. A monoclonal antibody based affinity column had been explored for the purpose of shortening the length of time needed for dioxin analysis and to decrease the amount of solvent consumption. The column retention pattern showed that the IAC binds the most toxic congeners in the dioxin and dibenzofuran series. Approximately 80% of total TEQ was retained by the column. Using carbon column in place of post IAC extraction makes the IAC potentially easy to automate.

Last Modified: 4/19/2014
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