|Nadella, Durga - NORTH DAKOTA STATE UNIV.|
Submitted to: Weed Science Society of America Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: September 21, 2000
Publication Date: February 14, 2000
Citation: Nadella, D.K., Foley, M.E. 2001. Developing a frame-work AFLP linkage map for dormancy investigations in wild oat (Avena fatua). [Abstract]. Weed Science Society of America Meeting. 41:21. Technical Abstract: Dormancy plays an important role in the persistence of wild oat rendering most of the weed control measures ineffective. Dormancy is a complex trait governed by many genes, with a large influence of environment on both the inception and the duration of the dormancy. A knowledge of the underlying genetic and environmental factors associated with dormancy and their interactions helps in devising an effective management strategy for wild oat. Molecular markers are proving to be useful tools in locating the loci underlying the complex traits such as seed dormancy in many important crops like rice and wheat. Mapping a complex polygenic trait in a hexaploid with a large genome like wild oat needs a marker technology that can target many loci simultaneously. The recently developed amplified fragment length polymorphism (AFLP) is the most efficient multilocus technology for generating greater number of polymorphisms in a short period of time. We are using AFLPs for mapping the quantitative trait loci (QTLs) for dormancy in a recombinant inbred (RI) population derived from the biparental cross of M73 (highly dormant) and SH430 (highly non-dormant) in wild oat. Two hundred forty- one polymorphisms were generated between M73 and SH430 with 23 primer combinations. Even though the percent polymorphisms was not high (16.5%), the ability of the technology to sample many loci simultaneously (63.5 per primer combination) resulted in a greater number of polymorphisms per primer combination (10.5) and a greater number of total polymorphisms. Additional markers will be generated and an AFLP linkage map will be constructed in the RI population with at least 600 markers.