|Bent, Andrew - UNIV MADISON, WISCONSIN|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 29, 2000
Publication Date: N/A
Technical Abstract: Arabidopsis rosettes resistant to P. syringae had increased levels of sinapic acid in the acetone extractable fraction from the cell wall residue 12 h post inoculation, which may have a role in the Hypersensitive Resistance (HR) signaling response. The relative abundance of sinapic acid in the control plants was similar to that found in the susceptible (HR-) plants. Arabidopsis rosettes susceptible to P. syringae had decreased levels of sinapyl alcohol in the water extractable fraction from the cell wall residue 36 h post inoculation, which may be due to the increased utilization of sinapyl alcohol pools in their conversion to syringyl lignin. The relative abundance of sinapyl alcohol in the control plants was three times less than in the resistant plants. Arabidopsis rosettes and stems 36 h post inoculation with P. syringae had increased syringyl lignin determined by staining with the Maule reagent and decreased guaiacy:syringyl ratio indicative of an increase in syringyl units deposited in the secondary wall. Rosettes that showed a resistant response to P. syringae showed uniformly increased syringyl lignin detected using the Maule reagent, in contrast to susceptible rosettes that showed localized increases that related to leaf symptom development.