Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 11, 2000
Publication Date: N/A
Technical Abstract: Interleukin-13 is an anti-inflammatory mediator produced by T cells that also stimulates the differentiation of B cells. We sought to identify SNPs in the bIL-13 gene to eventually determine if specific haplotypes were associated with improved or diminished IL-13-associated immune responsiveness. Polymorphisms were initially identified in 96 individuals representing 17 cattle breeds popular in commercial cattle operations in the United States. The complete MARC reference population was then screened in order to verify the SNPs by segregation using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We identified seven candidate SNPs within a 629 bp amplicon of the bIL-13 gene flanking and containing exon 1. Three out of the seven SNPs were located within the coding region of exon 1, and in all cases, translation analysis demonstrated that allelic substitution resulted in amino acid alteration. It is possible that these amino acid substitutions result in changes in cytokine activity, or possibly inactivation. Identification of SNP haplotypes for bIL-13 contributes to our pool of SNP markers for immune response genes in cattle, and will assist in the design of studies to determine the contribution of allelic variation to immunity.