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United States Department of Agriculture

Agricultural Research Service

Title: DNA Fingerprinting in Theobroma Cacao, the Chocolate Tree

Authors
item Saunders, James
item Mischke, Barbara
item Hemeida, Alaa - GEBRI
item Pedroni, Monica

Submitted to: PSNA Conference on Regulations of Phytochemicals by Molecular Techniques
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 2000
Publication Date: N/A

Technical Abstract: AFLP patterns from various Theobroma cacao genotypes were determined to establish relationships among populations of trees in germplasm collections. DNA fragments were selectively amplified, labeled with fluorescent dyes, and separated by capillary electrophoresis after selective PCR amplification. The peaks corresponding to DNA fragment patterns were reproducible and consistent within a common genotype. A similarity dendrogram was produced based on the combined analysis of multiple AFLP primer sets of polymorphic peaks between 50 and 400 base pairs detected from electropherograms using a PE/ABI 310 DNA analyzer. AFLP analyzes by this procedure defines individual accessions, identifies duplications of genotypes within germplasm collections, and establishes genetic similarity of breeding lines for potential crosses. This information allows for selection of appropriate germplasm stocks to be used din Theobroma cacao breeding programs to produce cultivars with improved tolerance/resistance to fungal diseases.

Last Modified: 8/21/2014
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