Submitted to: Society for Leukocyte Biology Meetings Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: August 2, 2000
Publication Date: N/A
Technical Abstract: Infectious disease in cattle is a serious health problem for humans and cattle, and results in significant economic loss to cattle producers. Resistance to infectious disease involves multiple genes, many of which are predicted to be mediators or effectors of the immune response. The purpose of our research is to identify cattle highly susceptible to infectious disease by attempting to use DNA markers such as single nucleotide polymorphisms (SNPs). SNPs are useful as gene-association markers in cattle because of their abundance, low mutation rates as compared to microsatellite markers, and amenability to automated analysis. To this end, we have identified four novel SNPs in the bovine interleukin-10 gene (IL-10). SNPs in the IL-10 gene were identified by sequencing representative unrelated individuals from 17 animal breeds representing over 95 percent of germplasm in use in the U.S. cattle industry. The identified allelic variation at this loci can now be tested for associatio with the ability of cattle to respond to infectious pathogens.