ARS | Publication request: PRODUCTION OF NEUTRALIZING ANTIBODY TO ALV-J DIFFERS IN AGED HENS FROM SEVEN B-CONGENIC WHITE LEGHORN LINES

Submitted to: International Symposium On Alv J
Publication Type: Abstract Only
Publication Acceptance Date: June 5, 2000
Publication Date: N/A

Technical Abstract: Eight to eleven 18-month old hens from seven 15.B congenic lines were immunized five times with line 0 CEF infected with ALV-J (Hc1). The plasma of all but one hen contained Hc1 one week post-immunization. The most sensitive and reliable measure of virus neutralizing (VN) antibody was 50 percent of the absorbency value of Hc1 infected CEF. VN antibody was not detected at three weeks post-immunization. At twenty four weeks all but tw hens had VN antibody, indicating the schedule of immunization was successful. VN antibody titers differed between lines, indicating the B haplotype may influence antibody response to Hc1. Hens with B*21 were more immunoresponsive to Hc1 than hens with B*2, B*5 or B*12. The results need to be verified for these lines, and extended to other chicken lines with comparable B* haplotypes, using Hc1 as well as other strains of ALV-J.