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United States Department of Agriculture

Agricultural Research Service

Title: Analysis of Serum Antibody to Alv-J in Commercial Broilers: Correlation Between Elisa, Flow Cytometry and Virus Neutralization

item Hunt, Henry
item Leathers, Valery - IDEXX LABORATORIES
item Lamichhane, C - KIRKEGAARD & PERRY LABS
item Rosales, Greg - ROSS BREEDERS
item Jensen, E - ROSS BREEDERS
item Mckay, J - ROSS BREEDERS
item Fadly, Aly

Submitted to: International Symposium On Alv J
Publication Type: Proceedings
Publication Acceptance Date: June 5, 2000
Publication Date: N/A

Technical Abstract: Virus neutralization, flow cytometry and ELISA tests were used to screen adult chicken sera for antibody to the avian leukosis subgroup J virus (ALV-J). The three methods employed gp85 (envelope proteins) from two different viral subtypes (HPRS-103 and ADOL-Hc1) for ALV-J antibody detection. Sera from virus positive and negative breeder flocks were screened. Four types of sera were observed using flow cytometry. The type sera were negative for the Hc1 and HPRS-103 envelopes. Type II sera were strongly positive for cells expressing both the Hc1 and the HPRS-103 envelopes. Type III sera was strongly positive for the HPRS-103 envelope but weak for cells expressing Hc1 and conversely, Type IV sera was strongly positive for the Hc1 envelope but weak for cells expressing the HPRS-103 envelope. The reactivity of Type II sera was similar (positive) in all the tests but the results of Types I, III and IV were variable. The high degree eof antigenic variation observed in the different ALV-J field isolates is suggested to be partially responsible for the poor correlation within and between the different assays compared.

Last Modified: 4/18/2015
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