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United States Department of Agriculture

Agricultural Research Service

Title: Method Validation by Ring Testing to Establish International Standards for Seed Testing; a Case Study

Authors
item Gooden, Jan - SARDI SOUTH AUSTRALIA
item SAMAC, DEBORAH
item Caffier, David - DRAF PAYS LOIRE FRANCE
item Ophel-Keller, Kathy - SARDI SOUTH AUSTRALIA
item Sheppard, James - CENTRAL SEED LAB CANADA

Submitted to: Plant Pathogenic Bacteria International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: July 24, 2000
Publication Date: N/A

Technical Abstract: ISTA has initiated a ring test to compare methods for the detection of Clavibacter michiganense subsp. insidiosum (Cmi), the causal agent of bacterial wilt in lucerne, to establish an international reference for the detection of Cmi in lucerne seed. Test plans and kits are submitted to participating laboratories to collect criteria for method validation. Various technologies are considered such as selective media plating, enzym immunoassay (ELISA), metabolic fingerprinting, and polymerase chain reaction (PCR). Test data are reviewed by the ISTA Plant Disease Committee (PDC). Parameters of sensitivity and specificity for the detection of Cmi in lucerne seed are set by the PDC in consultation with regulatory bodies and the seed industry.

Last Modified: 8/19/2014
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