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United States Department of Agriculture

Agricultural Research Service

Title: Osmolytes Enhance Room Temperature Shelf-Life of Freeze-Dried, Cells of Thebiocontrol Agent Enterobacter Cloacae S11:t:07 (Nrrl 210505)

item Van Cauwenberge, James
item Schisler, David
item Slininger, Patricia

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 20, 2000
Publication Date: N/A

Technical Abstract: Enterobacter cloacae S11:T:07 (NRRL 21050) has been identified as an antagonist effective against the causative agent of potato dry rot (Gibberella pulicaris) during tuber storage. In an effort to improve the shelf life of cells stored at room temperature, 15 osmolytes at 4 concentrations were added to individual E. cloacae cultures at initial inoculation or after 72 hours of incubation. Cultures were harvested at 9 hours incubation, freeze dried, stored at room temperature, and tested for culture viability over 21 days. Five osmolytes were identified that improved the shelf life of E. cloacae. Cell counts for control samples decreased from 10**10 to 10**1 CFU/ml by day 5 and approached 0 by day 7. In comparison, day 7 cultures which had turanose or arginine added at initial inoculation were at 10**8 CFU/ml and 10**5 CFU/ml, respectively. Day 7 cultures which had proline, maltose, or sucrose added at 72 hours were at 10**5 CFU/ml, 10**4 CFU/ml, or 10**6 CFU/ml, respectively. Furthe experimentation will be conducted to optimize product shelf life and determine the influence of osmolytes on cell efficacy.

Last Modified: 4/21/2015
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