Submitted to: Antonie Van Leeuwenhoek
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 27, 2000
Publication Date: N/A
Interpretive Summary: Foods and beverages are commonly spoiled by yeasts resulting in large economic losses to producers and consumers. Effective control of spoilage is dependent on accurate identification of the spoilage microorganisms so that detection methods can be developed. Apple juice and cider are often spoiled by yeasts, and in this research, DNA sequences (genetic material) were compared to identify cider spoilage yeasts that could not be identified using conventional growth tests. The DNA sequence comparisons showed that the spoilage yeasts represent three new species that have been named Candida anglica, Candida cidri and Candida pomiphila. The DNA sequences used for identification can form the basis for diagnostic probes to detect these spoilage yeasts.
Technical Abstract: The three new anamorphic ascomycetous yeasts Candida anglica (type strain NRRL Y-27079, CBS 4262), Candida cidri (type strain NRRL Y-27078, CBS 4241), and Candida pomiphila (type strain NRRL Y-27083, CBS 4242) are described. These three species were isolated from cider produced in the United Kingdom, and their identification was determined from unique nucleotide sequences in the species-specific D1/D2 domain of large subunit (26S) ribosomal DNA. Phylogenetic analysis of D1/D2 sequences placed C. anglica near Candida fragi, C. cidri near Pichia capsulata, and C. pomiphila in the Pichia holstii clade.