ARS | Publication request: A MODIFIED METHOD FOR SIMPLE, RAPID HPLC ANALYSIS OF LYCOPENE ISOMERS

Submitted to: Acta Horticulture Proceedings
Publication Type: Proceedings
Publication Acceptance Date: June 10, 2000
Publication Date: January 20, 2001
Citation: Ishida, B.K., Ma, J.C., Chan, B.G., Bartley, G.E., Grossman, J. 2001. A modified method for simple, rapid hplc analysis of lycopene isomers. Acta Horticulture Proceedings. Vol. 542, p. 235-242.

Technical Abstract: We describe a simple, rapid method to separate and quantify beta-carotene and geometric isomers of lycopene from tomato fruit. Carotenoids from extracts of tomato fruit are separated and eluted, using a reversed-phase HPLC with a C30 column and a mobile phase of 40% MTBE, 50% MeOH, and 10% EtOAc. With this system, we can achieve sharp resolution of cis and trans isomers of lycopene within approximately 25 min instead of the longer, mor complex gradient procedures required by previously described methods.