|Lee, Lawrence - CORNELL UNIVERSITY|
|Palukaitis, Peter - SCOTTISH CROP RES INST|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 2000
Publication Date: N/A
Technical Abstract: The potato leafroll virus (PLRV) 17 kDa protein (17K) has biochemical properties similar to several known viral movement proteins. The corresponding protein of Barley yellow dwarf virus is required for systemic infection of Avena sativa plants. However, the corresponding protein of Beet western yellows virus is dispensable for systemic infection of Nicotiana clevelandii, suggesting its role may be host dependent. To determine whether the PLRV 17K protein is involved in virus movement, ORF4, which encodes the 17K protein, was mutated by changing the double AUGs at the initiation codon to ACG. This mutation did not affect the protein sequence encoded by the overlapping coat protein gene. The mutant virus was inoculated onto N. clevelandii and N. benthamiana by Agrobacterium tumefaciens-mediated infection. The ORF4 mutation did not affect virus replication or assembly. Analysis of the agro-inoculated plants by ELISA indicated the ORF4 mutant did systemically infect both hosts and the PLRV 17K protein is not required for systemic movement.