ARS | Publication request: MICROSATELLITES IN MAIZE

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 17, 2000
Publication Date: March 17, 2000
Citation: SHAROPOVA, N., MCMULLEN, M.D., SCHULTZ, L., SCHROEDER, S., HOUCHINS, K.E., DAVIS, G., BERGSTROM, D., LISCUM, E., CONE, K., CHIN, E. MICROSATELLITES IN MAIZE - DEVELOPMENT AND MAPPING. MAIZE GENETICS CONFERENCE ABSTRACTS. 2000. ABSTRACT P. 88.

Technical Abstract: Simple sequence repeats (SSRs, microsatellites) are a class of tandem repeats that involve a base motif of 1-6 base pairs. In plants, SSRs are abundant, highly informative, and easy to use markers. Genome mapping using these sequence-derived markers will facilitate integration of physical and genetic maps in maize. One goal of the Missouri Maize Project is to develop pand map SSR markers and to release this information through MaizeDB. We ar developing SSR markers from both microsatellite-enriched genome libraries and systematic searching of publicly-available DNA sequences. Three mapping populations are exploited to map as many SSR polymorphisms as possible: IBM, F7:8 recombinant inbred population of Mo17 X B73 intermated through four generations, 360 individuals; TxCO IF2, immortalized F2 population of Tx303 X CO159, 54 individuals; and CEW6, immortalized F2 population of T218 X GT119, 93 individuals. Common SSR markers are mapped in all three populations to integrate information. Mapping on the IBM population will provide enhanced resolution for tightly linked loci while employment of IF2 and CEW6 will allow consolidation of newly-developed maps with current information.