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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Origin Between Two Bloodlines of Romosinuano Cattle Using Microsatellite Screening

item Chase, Chadwick
item Olson, T - UNIVERSITY OF FL
item Rohrer, Gary
item Coleman, Samuel

Submitted to: International Conference on Animal Genetics
Publication Type: Abstract Only
Publication Acceptance Date: July 22, 2000
Publication Date: July 22, 2000

Technical Abstract: Two primary bloodlines of Romosinuano cattle exist (i.e., a Costa Rican [COR] and a Colombian [COL] bloodline) and have been imported for eval- uation at STARS near Brooksville, Florida. The two bloodlines are pheno- typically indistinguishable except for occasional scurs or rare white spotting observed in the COR bloodline. The objective of this study was to odetermine whether or not the genetic variation between the two bloodlines was significant and how accurately an individual's bloodline of origin could be determined based on genetic markers. Forty-seven individuals from each bloodline were identified and screened across 46 microsatellite loci selected by proximity to published carcass merit QTL, usefulness in previously published genetic distance studies, or chromosomal location maximizing genomic coverage. Unique alleles (n=66, COR and n=47, COL) were detected in 41 of the microsatellite systems comprised a maximum allele frequency of 0.511 for a single system. Polymorphism information content values (both maximum and average) and average heterozygosities for the 46 systems were (COR) 0.891, 0.752, 0.571 and (COL) 0.918, 0.705, 0.579, respectively, facilitating correct bloodline assignments.

Last Modified: 4/22/2015