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United States Department of Agriculture

Agricultural Research Service

Title: Expression of Genens Involved in the Resistance of Soybean to Scn

Authors
item Matthews, Benjamin
item Beard, Hunter
item Macdonald, Margaret
item Alkharouf, Nadim - GEORGE MASON UNIVERSITY

Submitted to: International Congress of Plant Molecular Biology
Publication Type: Abstract Only
Publication Acceptance Date: April 7, 2000
Publication Date: N/A

Technical Abstract: The soybean cyst nematode (SCN), Heterodera glycines, is the major pest of soybean worldwide and is responsible for damage estimated at $1.5 million each year in the U.S. We are identifying genes involved in the resistance response of soybean to SCN using array techniques. Over 1000 ESTs were sequenced from a cDNA library constructed from cv. Peking mRNA two days after invasion of roots by SCN race 3. Numerous genes were identified by BLAST searches, including genes involved in secondary metabolism, signaling, and the defense response. Several genes involved in phenylpropanoid synthesis were found including phenylalanine ammonia lyase, chalcone synthase, cinnamyl alcohol dehydrogenase, 4-coumarate coA ligase, and others. Numerous peroxidases, protein kinases and transcription factors also were identified. Insert sizes range from ca. 400 bp to over 3.2 kb. Some clones were added to the database from differential display experiments that identified SCN-induced soybean genes. A set of 96 clones also was added from a cDNA library made from cotyledons to serve as non-induced genes in the array. Inserts of several clones are completely sequenced and contain complete reading frames: these are from genes that were available in this or other USDA laboratories. The database containing clone information is searchable by clone address, identity and size. The database can be viewed by collaborators at http://bldg6.arsusda.gov/benlab. A subset of the array is being hybridized to cDNA made from mRNA representing genes expressed in resistant and susceptible soybean inoculated and not inoculated with SCN to identify genes greatly induced in response to SCN. Benjamin F. Matthews, 301-504-5730, bmatthew@asrr.arsusda.gov

Last Modified: 4/18/2014
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