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United States Department of Agriculture

Agricultural Research Service

Title: Ribotyping for the Differentiation of Clostridium Perfringens Isolates from Poultry Production and Processing

item Craven, Stephen
item Stern, Norman
item Bailey, Joseph
item Cox, Nelson
item Cray, Paula

Submitted to: Poultry Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 30, 2000
Publication Date: N/A

Technical Abstract: Clostridium perfringens has been isolated from the poultry intestinal tract and processed carcasses. Little is known about sources of this poultry/human pathogen in the poultry operation. Samples were collected on two farms of a broiler chicken integrator every two weeks of a production cycle and from associated transport/ processing operations in each of four seasons providing 2945 samples. Results of ribotyping the Cp isolates are presented. Of 256 Cp isolates typed, 112(43%) were unique ribotypes(Rt) and 151(57%) belonged to one of 27 muliple- isolate ribogroups (MIR's) for a total of 139 Rt. Numerous Rt were isolated from each farm and associated operations and each sampling period. Isolates from one or more of the 27 MIR's that were recovered from scald-tank water, chill-tank water and carcass rinse samples after processing birds most often matched isolates recovered from previously collected samples of paper pads used to transport chicks from the hatchery, from coops before/after transport of birds to the processing plant, or from scald tank water samples taken before processing. Results indicate diversity among Cp isolates and the persistence and ubiquity of some Rt in the integrated operation. Production, transportation, and processing facilities may contribute to transmission of Cp to processed carcasses.

Last Modified: 4/21/2015
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