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United States Department of Agriculture

Agricultural Research Service

Title: CYTOPLASMIC MALE STERILITY IN PI 406647, PI 413024 AND PI 413158, AND THE INHERITANCE OF FERTILITY RESTORATION

Author
item JAN, CHAO-CHIEN

Submitted to: Proceedings Sunflower Research Workshop
Publication Type: Proceedings
Publication Acceptance Date: February 29, 2000
Publication Date: N/A

Interpretive Summary: Use of a single cytoplasmic male-sterile (cms) H. petiolaris cytoplasm (PET1) has promoted an intensive search for new cms and fertility restoration sources in wild Helianthus species and cultivated lines. The majority of the 62 FAO cms sources were from H. annuus accessions, and nine have restoration genes identified. However, determining the inheritance of fertility restoration genes lags far behind their initial identification and slows their utilization in commercial hybrid production. This paper reports the identification of three new cms sources in PI 406647, PI 413024, and PI 413158, and the identification and inheritance of their fertility restoration genes. Fertility restoration genes were identified for all the three cms sources. The inheritance of fertility restoration was controlled by single dominant genes for PI 406647 and PI413024, and further evaluations are needed for PI413158. These new cms can be considered as additional cms sources for sunflower breeding.

Technical Abstract: Male-sterile (MS) plants without extruding anthers and no pollen production were identified in populations of wild H. annuus PI 406647, PI 413024, PI 413043, and PI 413158. MS plants of each accession pollinated and backcrossed with HA89 3-5 times were crossed with 22 restoration testers in 1997. Male-fertile (MF) progenies in 1998 were self-pollinated and testcrossed onto respective advanced cms lines in HA89 background. Fertility restoration genes for both cms-PI 406647 and cms-PI 413024 were identified in the cultivated lines Issanka and Hopi Dye. Male-fertile plants in each of the original PI population also possessed fertility restoration gene(s) for the cms of the three PI's. The segregation ratios of 3 MF: 1 MS in F2 and 1MF: 1 MS in testcross progeny for genes restoring cms-PI 406647 and PI413024 suggested single dominant gene control of fertility restoration. The inheritance of restoration genes for cms-PI 413158, with the F2 results supporting single-dominant-gene hypothesis and the testcross progeny suggesting 2-complementary-gene control, requires further study. All three cms sources had over 96% seed set from sib- pollination and 0% seed set from self-pollination, indicating full female fertility and male sterility.

Last Modified: 8/27/2014
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