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United States Department of Agriculture

Agricultural Research Service

Title: Gene Expression in Tomato Fruit Engineered to Accumulate Polyamines During Ripening

Authors
item Cassol, Tatiana
item Mehta, Roshni - FORMERLY OF VEGETABLE LAB
item Ali, Nasreen - UNIVERSITY OF MARYLAND
item Handa, Avtar - PURDUE UNIVERSITY
item Mattoo, Autar

Submitted to: International Society for Horticultural Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2000
Publication Date: N/A

Technical Abstract: Tomato fruits were engineered to accumulate polyamines, spermidine and spermine, during ripening by genetic introduction of yeast S-adenosylmethionine decarboxylase gene fused with the ripening-and ethylene-inducible E-8 promoter. Beneficial attributes of the transgenic fruit include longer vine and shelf life, robustness and a 3-fold increase in the lycopene content. These fruit consistently produced more ethylene than the azygous fruit. This in vivo observation is in contrast to our previous in vitro studies where incubation of tomato fruit slices with mM concentrations of spermidine and spermine inhibited accumulation of ACC synthase transcripts. We tested whether the E-8 promoter sequences in the gene construct co-suppressed the endogenous E8 gene, which is an ethylene biosynthesis suppressor. Northern blot analysis negated this possibility. Further, we analyzed accumulation of transcripts for ACC synthase, ACC oxidase, etr3, actin, E-8, and rDNA in transgenic and non-transgenic fruits at different stages of ripening. These data will be presented and discussed in relation to metabolic interactions between polyamines and ethylene in climacteric fruits.

Last Modified: 9/20/2014
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