Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Isolation of 31 New Porcine Microsatellites from a Microsatellite Enriched Microdissected Chromosome 8 Library

Authors
item Wang, Z - KANSAS STATE UNIV
item Rohrer, Gary
item Stone, Roger
item Troyer, Deryl - KANSAS STATE UNIV

Submitted to: Animal Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 1, 2000
Publication Date: June 12, 2000

Interpretive Summary: A method was developed in which scientists could develop genetic markers for specific chromosomes. The method was demonstrated by targeting pig chromosome 8 and isolating 31 genetic markers. Fifty-five percent of the markers developed mapped to pig chromosome 8 and most of the rest of the markers mapped to pig chromosome 15. Apparently portions of pig chromosome e15 were also selected during the isolation process. Pig chromosome 8 was selected as it has been identified as containing genes affecting litter size in pigs. This new method to develop genetic markers is of great importance to the entire mapping community as it will improve the efficiency of marker development in areas where important genes are located and facilitate finding the actual gene of interest. The method will be used in livestock genetics to increase the number of markers surrounding genes affecting economically important traits.

Technical Abstract: Chromosome 8 is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci. The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer PCR. The PCR product was then enriched for (CA)n microsatellites by hybridization to a biotinylated CA repeat oligonucleotide and captured by streptavidin-coated magnetic beads. The captured DNA was cloned into a TA cloning vector. Screening with an isotopically labeled CA oligonucleotide probe resulted in the isolation of 31 informative and polymorphic microsatellite clones. Seventeen of those were mapped to chromosome 8, 12 to chromosome 15, 1 to chromosome 10 and 1 to chromosome X. The markers were all placed on the USDA-MARC porcine genetic linkage map.

Last Modified: 8/22/2014