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United States Department of Agriculture

Agricultural Research Service

Title: Evaluation of a Lateral Flow Device for Detecting Salmonella Enteritidis in Raw Eggs and Chicken Feces

Authors
item Seo, Kun Ho
item Holt, Peter
item Mitchell, Bailey
item Gast, Richard

Submitted to: Milk Food and Environmental Sanitarians International Association
Publication Type: Abstract Only
Publication Acceptance Date: April 7, 2000
Publication Date: July 24, 2000
Citation: Seo, K., Holt, P.S., Mitchell, B.W., Gast, R.K. 2000. Evaluation of a lateral flow device for detecting salmonella enteritidis in raw eggs and chicken feces. Milk Food and Environmental Sanitarians International Association.

Technical Abstract: A lateral flow device for the detection of Salmonella Enteritidis (SE) has been developed. The test panel is a presumptive qualitative test system that detects only members of Group D1 Salmonella species. Detection of SE was 100% in raw egg pools inoculated with SE 1-10 CFU/ml of egg and incubated in buffered peptone water (BPW) or tetrathionate broth (TT) at 1:10 ratio for 24 h at 37C. The panels detected 100% of chicken manure samples initially seeded with 1 CFU SE/g and enriched in tetrathionate broth for 24 h at 37C. SE did not grow as well in straight cecal samples and, in many cases, after 24 enrichment did not attain the minimal levels of organism detectable by the panels. The false positive ratio was less than 0.04% in the fecal samples from chickens infected with Salmonella Typhimurium or Kentucky. The test kit detected 94% and 100% of enriched fecal samples from SE-infected birds at 4 and 7 days post infection (PI), respectively, but detection decreased to 62% and 83% at 14 and 20 days PI. The developed lateral flow test kit could provide a simple, rapid, and inexpensive method for egg producers and processors to test specifically for Salmonella group D1 serovars, such as SE, in environmental and egg samples.

Last Modified: 8/22/2014
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