Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 1999
Publication Date: June 1, 1999
Citation: GROSZ, M.D., FAHRENKRUG, S.C., MACNEIL, M.D. COMPARATIVE POSITIONAL CANDIDATE CLONING: KIT AS A CANDIDATE GENE FOR THE HEREFORD COAT COLOR PHENOTYPE. PLANT AND ANIMAL GENOME CONFERENCE PROCEEDINGS. v. 42. p. 160-162. 1999. Technical Abstract: The spotted locus is responsible for several phenotypically distinguishable piebald patterns in cattle, including Hereford, or white face (S**H), lineback (S**P), and recessive spotting (s), in addition to non-spotted (S**+). Coat color has been implicated in predisposition to Bovine Ocular Squamous Cell Carcinoma ("cancer eye") and sunburned udders, both of which occur at higher frequencies in Hereford populations. In a backcross mapping population, the S locus has been mapped by genetic linkage to bovine chromosome 6, showing complete linkage with microsatellite marker CA028. A Single Nucleotide Polymorphism within intron 15 of the Kit gene has also been mapped by linkage to chromosome 6, linked to CA028. This region corresponds comparatively to a region on mouse chromosome 5 responsible for several coat color mutations including kit, patch and rump-white. These mutations in mice have been ascribed to dysfunctional expression of the genes encoding the receptor tyrosine kinases c-kit and platelet-derived growth factor receptor alpha chain (PDGFRA). The phenotype and mode of inheritance of the bovine S locus is reminiscent of allelic variation at these murine loci. Data is presented indicating genetic linkage between the bovine S locus and microsatellite markers physically proximal to c- Kit on chromosome 6.