Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: August 19, 1999
Publication Date: N/A
Technical Abstract: DNA sequence variation provides the fundamental material for improving livestock through selection. Single nucleotide polymorphisms and small insertion/deletions (collectively referred to here as SNPs) represent a potential resource for analyzing DNA sequence variation in cattle. SNPs have become the markers of choice in human genetic studies because of their rhigh frequency, low mutation rates, and amenability to automated analysis. In cattle we have identified SNPs in genes, scored them in a reference population, and determined their linkage map position. However, for SNP marker strategies to be broadly applied in bovine genetic studies, an estimate of their frequency and information content is required along with automated means of detection. The present study measured bovine DNA sequence variation and haplotype diversity and evaluated automated high-throughput genotyping in cattle by matrix-assisted laser description/ionization time-of-flight mass spectrometry (MALDI-TOF MS).