Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: April 28, 2000
Publication Date: N/A
Technical Abstract: Pseudomonas aeruginosa strain PR3 converts ricinoleic acid to 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD). The mechanism of TOD formation via an intermediate 10,12- dihydroxy-8(E)-octadecenoic acid involves one hydration, a double bond rearrangement, and one hydroxylation of the substrate molecule. However, strain PR3 was unable to convert lesquerolic acid to a comparable multi-hydroxy product. The bioconversion of ricinoleic acid was always higher in rich WF media than in minimal screening media. Production of TOD was sensitive to the reaction time. Extending the conversion time from 48 to 72 h prior to lipid extraction decreased the yield of TOD from 20.0% to 2.3%. By further defining culture growth conditions, reaction pH and temperature, and substrate concentration, the maximal yield of TOD production was over 35%.