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United States Department of Agriculture

Agricultural Research Service

Title: A Comparison of the Immunological Response to Lipopolysaccharide (Lps) Versus E. Coli Challenge in the Weaned Pig

Authors
item Zannelli, M - ENDOGEN, INC.
item Touchette, K - UNIVERSITY OF MISSOURI
item Allee, G - UNIVERSITY OF MISSOURI
item Matteri, Robert
item Beausang, L - ENDOGEN, INC.
item Luchene, L - ENDOGEN, INC.
item Carroll, Jeffery

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2000
Publication Date: N/A

Technical Abstract: This study evaluated potential differences in immunological responses between an LPS and E. coli challenge in the weaned pig. Forty weaned pigs (14 d, 5 kg) were used in 2 experiments (EXP 1 and 2). In EXP 1, pigs were allotted to 1 of 2 treatments: 1) Cont; i.p. injection of saline or 2) i.p. injection of LPS (150 ug/kg BW). In EXP 2, pigs were also allotted to 1 of f2 treatments: 1) Cont; oral dose of sterile culture media (5 ml) or 2) 5 m oral dose of E. Coli (100 million CFU/ml). Starter rations were fed for 7 d prior to immunological challenges. On d 6 post weaning, all pigs were non-surgically fitted with a jugular cannulae. On d 7 of EXP 1, pigs received their respective i.p. treatments followed by blood sampling every 15 min for 3 h. On d 7 of EXP 2, pigs received their respective treatments followed by blood sampling for 10 h. Serum TNF-alpha and IFN-gamma were assessed by porcine specific ELISAs. In the LPS challenged group, there was a time x treatment effect for serum TNF-alpha (P<.0001) and IFN-gamma (P<.0001), where the first significant difference in serum TNF-alpha was at 0.75 h with 1.38 ng/ml (P=.01). Peak TNF-alpha was 10.85 ng/ml at 1.25 h post-LPS. The first significant difference in serum IFN-gamma was at 1.75 h with 187.1 pg/ml (P=.01). Peak IFN-gamma was 995.4 pg/ml at 3 h. In the E. Coli challenged group, there was no difference in the serum TNF-alpha, however, there was a significant time x treatment difference in serum IFN-gamma (P<.001). There was a trend for E. coli to increase serum IFN-gamma beginning at 5 h compared to controls (P=.14). Peak IFN-gamma was 26.2 pg/ml at 7 h and remained at that level for the duration of the study. These results demonstrate that LPS and E. coli challenges induce very different immunological profiles in the weaned pig.

Last Modified: 7/25/2014
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